Article
[Cloning and sequencing of KIR2DL1 framework gene cDNA and identification of a novel allele].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Oct 2016
Sun Ge, Wang Chang, Zhen Jianxin, Zhang Guobin, Xu Yunping, Deng Zhihui
Abstract excerpt
OBJECTIVE: To develop an assay for cDNA cloning and haplotype sequencing of KIR2DL1 framework gene and determine the genotype of an ethnic Han from southern China. METHODS: Total RNA was isolated from peripheral blood sample, and complementary DNA (cDNA) transcript was synthesized by RT-PCR. The entire coding sequence of the KIR2DL1 framework gene was amplified with a pair of KIR2DL1-specific PCR primers. The PCR...
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