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Article

Generation of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing.

Biotechnology letters - 1 Dec 2016

Chung Woo Young, Song Myungjae, Park Jinhong, Namkung Wan, Lee Jinu, Kim Hyongbum, Lee Min Goo, Kim Joo Young

Abstract excerpt

OBJECTIVES: To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS: CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels that abolished CFTR protein expressions. Next, stable ΔF508-CFTR expression was achieved by...

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Source

Identifiers

PMID: 27571970
DOI: 10.1007/s10529-016-2190-4

Genes

CFTR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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