Article
Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley.
BioMed research international - 1 Jan 2016
Lu Ruiju, Chen Zhiwei, Gao Runhong, He Ting, Wang Yifei, Xu Hongwei, Guo Guimei, Li Yingbo, Liu Chenghong, Huang Jianhua
Abstract excerpt
To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease...
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