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Article

Improving the efficiency of isolated microspore culture in six-row spring barley: I-optimization of key physical factors.

Plant cell reports - 1 Jun 2014

Esteves Patricio, Belzile François

Abstract excerpt

An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just a few months via androgenesis in vitro. DHs are useful tools in genetic research and in plant...

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PMID: 24563120
DOI: 10.1007/s00299-014-1583-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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