Article
A FRET-based method for monitoring septin polymerization and binding of septin-associated proteins.
Methods in cell biology - 1 Jan 2016
Booth E A, Thorner J
Abstract excerpt
Much about septin function has been inferred from in vivo studies using mainly genetic methods, and much of what we know about septin organization has been obtained through examination of static structures in vitro primarily by electron microscopy. Deeper mechanistic insight requires real-time analysis of the dynamics of the assembly of septin-based structures and how other proteins associate with them. We...
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