Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with <i>Bordetella bronchiseptica</i> Infection

Abstract excerpt

BACKGROUND: The use of quantitative PCR (qPCR) for detection of Bordetella bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. Coinfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not...

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