Article
Multiplexed steroid profiling of gluco- and mineralocorticoids pathways using a liquid chromatography tandem mass spectrometry method.
The Journal of steroid biochemistry and molecular biology - 1 Jan 2017
Travers Simon, Martinerie Laetitia, Bouvattier Claire, Boileau Pascal, Lombès Marc, Pussard Eric
Abstract excerpt
Serum steroid assays are major tools in the clinical evaluation of adrenal disorders. The main adrenal steroids are routinely measured with immunoassays. However, chromatographic methods are known to offer better specificity. We report a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous quantification of 15 adrenal steroids targeting the mineralo- and gluco-corticosteroid pathways....
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