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The Parasol Protocol for computational mutagenesis.

Protein engineering, design & selection : PEDS - 1 Jul 2016

Aronica P G A, Verma C, Popovic B, Leatherbarrow R J, Gould I R

Abstract excerpt

To aid in the discovery and development of peptides and proteins as therapeutic agents, a virtual screen can be used to predict trends and direct workflow. We have developed the Parasol Protocol, a dynamic method implemented using the AMBER MD package, for computational site-directed mutagenesis. This tool can mutate between any pair of amino acids in a computationally expedient, automated manner. To demonstrate...

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PMID: 27255759
DOI: 10.1093/protein/gzw009

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