Article
Whole-genome typing and characterization of blaVIM19-harbouring ST383 Klebsiella pneumoniae by PFGE, whole-genome mapping and WGS.
The Journal of antimicrobial chemotherapy - 1 Jun 2016
Sabirova Julia S, Xavier Basil Britto, Coppens Jasmine, Zarkotou Olympia, Lammens Christine, Janssens Lore, Burggrave Ronald, Wagner Trevor, Goossens Herman, Malhotra-Kumar Surbhi
Abstract excerpt
OBJECTIVES: We utilized whole-genome mapping (WGM) and WGS to characterize 12 clinical carbapenem-resistant Klebsiella pneumoniae strains (TGH1-TGH12). METHODS: All strains were screened for carbapenemase genes by PCR, and typed by MLST, PFGE (XbaI) and WGM (AflII) (OpGen, USA). WGS (Illumina) was performed on TGH8 and TGH10. Reads were de novo assembled and annotated [SPAdes, Rapid Annotation Subsystem...
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