Article
High-resolution phylogenetic microbial community profiling.
The ISME journal - 1 Aug 2016
Singer Esther, Bushnell Brian, Coleman-Derr Devin, Bowman Brett, Bowers Robert M, Levy Asaf, Gies Esther A, Cheng Jan-Fang, Copeland Alex, Klenk Hans-Peter, Hallam Steven J, Hugenholtz Philip, Tringe Susannah G, Woyke Tanja
Abstract excerpt
Over the past decade, high-throughput short-read 16S rRNA gene amplicon sequencing has eclipsed clone-dependent long-read Sanger sequencing for microbial community profiling. The transition to new technologies has provided more quantitative information at the expense of taxonomic resolution with implications for inferring metabolic traits in various ecosystems. We applied single-molecule real-time sequencing for...
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