Article
Development of a rapid and high-throughput molecular method for detecting the F200Y mutant genotype in benzimidazole-resistant isolates of Fusarium asiaticum.
Pest management science - 1 Nov 2016
Duan Yabing, Yang Ying, Li Tao, Zhao Donglei, Cao Junhong, Shi Yiyuan, Wang Jianxin, Zhou Mingguo
Abstract excerpt
BACKGROUND: The point mutation at codon 200 (TTC→TAC, F200Y) of the β2 -tubulin gene confers resistance to benzimidazole fungicide in Fusarium asiaticum. These isolates with this mutation have been detected mainly by determining the minimum inhibitory concentration (MIC) of fungicides, which is always time consuming, tedious and inefficient. RESULTS: A visual, rapid and efficient method with high specificity was...
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