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Article

Prenatal diagnosis of single gene disorders using amniotic fluid as the starting material for PCR.

The Analyst - 7 Jan 2016

Huang Huan, Li Shuo, Lu Shuolian, Ge Hongshan, Sun Lizhou

Abstract excerpt

A rapid and inexpensive method for fetal genetic diagnosis using amniotic fluid (AF) as the starting material was demonstrated in this study. Raw AF was added directly to polymerase chain reaction (PCR) mixtures with HpH buffer (a high pH buffer), without any pre-treatment. Amplified products were detected by gel electrophoresis and then subjected to Sanger sequencing. The AF from four fetuses, each expressing a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 26587643
DOI: 10.1039/c5an01840d

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