Article
A simple and powerful approach for isolation of Arabidopsis mutants with increased tolerance to H2O2-induced cell death.
Methods in enzymology - 1 Jan 2013
Gechev Tsanko, Mehterov Nikolay, Denev Iliya, Hille Jacques
Abstract excerpt
A genetic approach is described to isolate mutants more tolerant to oxidative stress. A collection of T-DNA activation tag Arabidopsis thaliana mutant lines was screened for survivors under conditions that trigger H2O2-induced cell death. Oxidative stress was induced by applying the catalase (CAT) inhibitor aminotriazole (AT) in the growth media, which results in decrease in CAT enzyme activity, H2O2...
Topics
- Adaptation, Physiological
- Anthocyanins
- Apoptosis
- Arabidopsis
- Arabidopsis Proteins
- Catalase
- Enzyme Assays
- Ethyl Methanesulfonate
- Genes, Plant
- Hydrogen Peroxide
- Malondialdehyde