Article
Multiplex SSR-PCR approaches for semi-automated genotyping and characterization of loci linked to blast disease resistance genes in rice.
Comptes rendus biologies - 1 Nov 2015
Ashkani Sadegh, Rafii Mohd Yusop, Shabanimofrad Mahmoodreza, Foroughi Majid, Azizia Parisa, Akhtar Mohd Sayeed, Sahebi Mahbod, Harun Abd Rahim, Nasehi Abbas
Abstract excerpt
In the present study, 63 polymorphic microsatellite markers related to rice blast resistance genes were fluorescently labelled at the 5'-end with either 6-FAM or HEX using the G5 dye set and incorporated into a multiplex SSR-PCR for the detection of fragments using an automated system. For rice F3 families obtained from crosses between Pongsu Seribu 2 (Malaysian blast resistant cultivar) and Mahsuri (a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
