Fast, sensitive and specific diagnosis of infections withLeishmaniaspp. in formalin-fixed, paraffin-embedded skin biopsies by cytochrome b polymerase chain reaction

Abstract excerpt

BACKGROUND: Northern spread of sandflies and Leishmania spp. has been observed in Europe. Diagnosis can be difficult owing to the various clinical manifestations. Species identification is important for patient management and therapy. Molecular diagnostics is increasingly used for pan-Leishmania detection but species identification remains challenging in formalin-fixed material. OBJECTIVES: To apply cytochrome b...

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