Article
ASG2 is a farnesylated DWD protein that acts as ABA negative regulator in Arabidopsis.
Plant, cell & environment - 1 Jan 2016
Dutilleul Christelle, Ribeiro Iliana, Blanc Nathalie, Nezames Cynthia D, Deng Xing Wang, Zglobicki Piotr, Palacio Barrera Ana María, Atehortùa Lucia, Courtois Martine, Labas Valérie, Giglioli-Guivarc'h Nathalie, Ducos Eric
Abstract excerpt
The tagging-via-substrate approach designed for the capture of mammal prenylated proteins was adapted to Arabidopsis cell culture. In this way, proteins are in vivo tagged with an azide-modified farnesyl moiety and captured thanks to biotin alkyne Click-iT® chemistry with further streptavidin-affinity chromatography. Mass spectrometry analyses identified four small GTPases and ASG2 (ALTERED SEED GERMINATION 2), a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
