Article
Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil.
Epidemiology and infection - 1 Jan 2016
Hamond C, Pestana C P, Medeiros M A, Lilenbaum W
Abstract excerpt
The aim of this study was to identify Leptospira in urine samples of cattle by direct sequencing of the secY gene. The validity of this approach was assessed using ten Leptospira strains obtained from cattle in Brazil and 77 DNA samples previously extracted from cattle urine, that were positive by PCR for the genus-specific lipL32 gene of Leptospira. Direct sequencing identified 24 (31·1%) interpretable secY...
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