Article
Negeviruses found in multiple species of mosquitoes from southern Portugal: Isolation, genetic diversity, and replication in insect cell culture.
Virology - 1 Sept 2015
Carapeta Sara, do Bem Beatriz, McGuinness James, Esteves Aida, Abecasis Ana, Lopes Ângela, de Matos António P, Piedade João, de Almeida António P G, Parreira Ricardo
Abstract excerpt
In this report, an RT-PCR approach based on the use of degenerate primers allowed the identification of negeviruses in four different species of mosquitoes (Ochlerotatus caspius, Culex pipiens, Cx. theileri and Cx. univittatus) collected in southern Portugal. The genomes of two of these viruses, sequenced to full completion, were shown to encode all the proteins encoded by previously described negeviruses. One of...
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