Article
Correlation Between Pneumocystis jirovecii Mitochondrial Genotypes and High and Low Fungal Loads Assessed by Single Nucleotide Primer Extension Assay and Quantitative Real-Time PCR.
The Journal of eukaryotic microbiology - 1 Jan 2000
Alanio Alexandre, Olivi Martine, Cabaret Odile, Foulet Françoise, Bellanger Anne-Pauline, Millon Laurence, Berceanu Ana, Cordonnier Catherine, Costa Jean-Marc, Bretagne Stéphane
Abstract excerpt
We designed a single nucleotide primer extension (SNaPshot) assay for Pneumocystis jirovecii genotyping, targeting mt85 SNP of the mitochondrial large subunit ribosomal RNA locus, to improve minority allele detection. We then analyzed 133 consecutive bronchoalveolar lavage (BAL) fluids tested positive for P. jirovecii DNA by quantitative real-time PCR, obtained from two hospitals in different locations (Hospital...
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