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The cell‐surface proteome of cultured adipose stromal cells

30 Apr 2015

Abstract excerpt

In this technical note we describe a method to evaluate the cell surface proteome of human primary cell cultures and cell lines. The method utilizes the BD Biosciences lyoplate, a system covering 242 surface proteins, glycoproteins, and glycosphingolipids plus relevant isotype controls, automated plate-based flow cytometry, conventional file-level analysis and unsupervised K-means clustering of markers on the...

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PMID: 25929697
DOI: 10.1002/cyto.a.22682

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