Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

doi:10.1038/s41598-018-29477-5

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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

Scientific reports - 27 Jul 2018

Mieczkowska Alina, Schumacher Adriana, Filipowicz Natalia, Wardowska Anna, Zieliński Maciej, Madanecki Piotr, Nowicka Ewa, Langa Paulina, Deptuła Milena, Zieliński Jacek, Kondej Karolina, Renkielska Alicja, Buckley Patrick G, Crossman David K, Crowley Michael R, Czupryn Artur, Mucha Piotr, Sachadyn Paweł, Janus Łukasz, Skowron Piotr, Rodziewicz-Motowidło Sylwia, Cichorek Mirosława, Pikuła Michał, Piotrowski Arkadiusz

Abstract excerpt

Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell...

Topics

Adipose Tissue
Biomarkers
Cell Differentiation
Cells, Cultured
Gene Expression Profiling
Gene Regulatory Networks
Humans
Immunophenotyping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Protein Isoforms

RNA, Messenger

Stem Cells

Transcription, Genetic

Transcriptome

Source

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Identifiers

PMID: 30054533
DOI: 10.1038/s41598-018-29477-5
PMCID: PMC6063933

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