Article
PFGE protocols to distinguish subspecies of Lactococcus lactis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Le Bourgeois Pascal, Passerini Delphine, Coddeville Michèle, Guellerin Maéva, Daveran-Mingot Marie-Line, Ritzenthaler Paul
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction...
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