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Article

Melting curve analysis of a groEL PCR fragment for the rapid genotyping of strains belonging to the Lactobacillus casei group of species.

Microbiological research - 1 Apr 2015

Koirala Ranjan, Taverniti Valentina, Balzaretti Silvia, Ricci Giovanni, Fortina Maria Grazia, Guglielmetti Simone

Abstract excerpt

Lactobacillus casei group (Lcs) consists of three phylogenetically closely related species (L. casei, L. paracasei, and L. rhamnosus), which are widely used in the dairy and probiotic industrial sectors. Strategies to easily and rapidly characterize Lcs are therefore of interest. To this aim, we developed a method according to a technique known as high resolution melting analysis (HRMa), which was applied to a...

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PMID: 25801971
DOI: 10.1016/j.micres.2015.01.001

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