Article
Functional analysis of (4S)-limonene synthase mutants reveals determinants of catalytic outcome in a model monoterpene synthase.
Proceedings of the National Academy of Sciences of the United States of America - 17 Mar 2015
Srividya Narayanan, Davis Edward M, Croteau Rodney B, Lange B Markus
Abstract excerpt
Crystal structural data for (4S)-limonene synthase [(4S)-LS] of spearmint (Mentha spicata L.) were used to infer which amino acid residues are in close proximity to the substrate and carbocation intermediates of the enzymatic reaction. Alanine-scanning mutagenesis of 48 amino acids combined with enzyme fidelity analysis [percentage of (-)-limonene produced] indicated which residues are most likely to constitute...
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