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Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1.

FEMS microbiology letters - 1 Jan 2015

Ayub Nicolás D, Fox Ana R, García Araceli N, Mozzicafreddo Matteo, Cuccioloni Massimiliano, Angeletti Mauro, Pagano Elba, Soto Gabriela

Abstract excerpt

Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three...

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PMID: 25670697
DOI: 10.1093/femsle/fnu006

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