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Random mutagenesis and overexpression of rhodopin-3,4-desaturase allows the production of highly conjugated carotenoids in Rhodospirillum rubrum.

Archives of biochemistry and biophysics - 15 Apr 2015

Autenrieth Caroline, Ghosh Robin

Abstract excerpt

The crtD gene of the purple bacterium Rhodospirillum rubrum, encoding rhodopin desaturase, was cloned into a broad-host range expression plasmid (pRKCAG53) and transferred to the R. rubrum crtD(-) mutant, ST4, which restored the wild-type phenotype and produced the carotenoid spirilloxanthin. pRKCAG53 was randomly mutated in an Escherichia coli mutator strain and then transferred to ST4 for selection of...

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PMID: 25655347
DOI: 10.1016/j.abb.2015.01.023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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