Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Random mutagenesis and overexpression of rhodopin-3,4-desaturase allows the production of highly conjugated carotenoids in Rhodospirillum rubrum.

Archives of biochemistry and biophysics - 15 Apr 2015

Autenrieth Caroline, Ghosh Robin

Abstract excerpt

The crtD gene of the purple bacterium Rhodospirillum rubrum, encoding rhodopin desaturase, was cloned into a broad-host range expression plasmid (pRKCAG53) and transferred to the R. rubrum crtD(-) mutant, ST4, which restored the wild-type phenotype and produced the carotenoid spirilloxanthin. pRKCAG53 was randomly mutated in an Escherichia coli mutator strain and then transferred to ST4 for selection of...

Topics

Carotenoids
Cloning, Molecular
Frameshift Mutation
Gene Expression
Genetic Engineering
Mutagenesis
Oxidoreductases
Phenotype
Rhodospirillum rubrum
Sequence Analysis, DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 25655347
DOI: 10.1016/j.abb.2015.01.023

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Random mutagenesis and overexpression of rhodopin-3,4-desaturase allows the production of highly conjugated carotenoids in Rhodospirillum rubrum.PMID 25655347Read ↗

A null lesion in the rhodopin 3,4-desaturase of Rhodospirillum rubrum unmasks a cryptic branch of the carotenoid biosynthetic pathway.Semantic proximity Read ↗

Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides.Semantic proximity Read ↗

Evaluation of colors in green mutants isolated from purple bacteria as a host for colorimetric whole-cell biosensors.Semantic proximity Read ↗

Photooxidative stress stimulates illegitimate recombination and mutability in carotenoid-less mutants of Rubrivivax gelatinosus.Semantic proximity Read ↗

Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma).Semantic proximity Read ↗

Structural and kinetics properties of a mutated phytoene desaturase from Rubrivivax gelatinosus with modified product specificity.Semantic proximity Read ↗

Rhodopseudomonas palustris CGA009 has two functional ppsR genes, each of which encodes a repressor of photosynthesis gene expression.Semantic proximity Read ↗

Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants.Semantic proximity Read ↗

Rhodospirillum rubrum possesses a variant of the bchP gene, encoding geranylgeranyl-bacteriopheophytin reductase.Semantic proximity Read ↗

Harnessing the catabolic diversity of rhodococci for environmental and biotechnological applications.Semantic proximity Read ↗

Spectroscopic analysis of genetically modified photosynthetic reaction centers.Semantic proximity Read ↗

Development of Xanthophyllomyces dendrorhous as a production system for the colorless carotene phytoene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.