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[Rapid PCR authentication Lonicera japanica].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Oct 2014

Jiang Chao, Hou Jing-Yi, Huang Lu-Qi, Yuan Yuan, Chen Min, Jin Yan

Abstract excerpt

To simply and rapid authenticate Lonicera japanica. Rapid allele-specific PCR primer was designed base on trnL-trnF 625 G/T Single nucleotide polymorphism and the PCR reaction systems including annealing temperature was optimized; optimized results were performed to authenticate L. japanica and its 9 adulterants. When 100 x SYBR Green I was added in the PCR product of 87 degrees C initial denatured 1 min; 87...

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PMID: 25612418

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