Article
[Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Dec 2012
Jiang Chao, Zhang Ya-Hua, Chen Min, Yuan Yuan, Lin Shu-Fang, Wu Zhi-Gang
Abstract excerpt
OBJECTIVE: To identify SNP in flos Lonicerae, and authenticate Lonicera japonica from its adulterants and the mixture by using bidirectional PCR amplification of specific alleles (Bi-PASA). METHOD: SNP of L. japonica and its adulterants was identified by using ClustulW to align trnL-trnF sequences of the Lonicera genus from GenBank database. Bi-PASA primer was designed and the PCR reaction systems including...
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