Article
[Application of rapid PCR to authenticate Ranae Oviductus].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Jul 2017
He Zhi-Yi, Tang Xian-Ming, Liu Jian-Hui, Yuan Yuan, Jiang Chao, Zhao Yu-Yang, Wang Yang
Abstract excerpt
Rapid allele-specific PCR primer was designed base on Cytb 155 A/T single nucleotide polymorphism, DNA was extracted by alkaline lysis and the PCR reaction systems including denatured and annealing temperature and cycle numbers were optimized. The results were performed to authenticate Ranae Oviductus and its 4 adulterants. When 100×SYBR Green I was added in the PCR product at 90 ℃ denatured 3 s, 62 ℃ annealing...
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