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Article

Mutants of Micromonospora viridifaciens sialidase have highly variable activities on natural and non-natural substrates.

Protein engineering, design & selection : PEDS - 1 Feb 2015

Jers C, Guo Y, Kepp K P, Mikkelsen J D

Abstract excerpt

This study aimed to improve the hydrolase activity of the well-characterised bacterial sialidase from Micromonospora viridifaciens. The enzyme and its mutated versions were produced in Bacillus subtilis and secreted to the growth medium. Twenty amino acid positions in or near the active site were subjected to site-saturation mutagenesis and evaluated on the artificial sialidase substrate...

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PMID: 25573534
DOI: 10.1093/protein/gzu054

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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