Article
Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability.
Bioscience, biotechnology, and biochemistry - 1 Jan 2015
Nishijima Hiroto, Nozaki Kouichi, Mizuno Masahiro, Arai Tsutomu, Amano Yoshihiko
Abstract excerpt
The xylanase (Xyn10B) that strongly adsorbs on microcrystalline cellulose was isolated from Driselase. The Xyn10B contains a Carbohydrate-binding module family 1 (CBM1) (IrpCBMXyn10B) at N-terminus. The canonical essential aromatic residues required for cellulose binding were conserved in IrpCBMXyn10B; however, its adsorption ability was markedly higher than that typically observed for the CBM1 of an...
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