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Article

Observation of superoxide production during catalysis of Bacillus subtilis oxalate decarboxylase at pH 4.

Free radical biology & medicine - 1 Mar 2015

Twahir Umar T, Stedwell Corey N, Lee Cory T, Richards Nigel G J, Polfer Nicolas C, Angerhofer Alexander

Abstract excerpt

This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin-trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid, the hydroperoxyl radical, is expected as an intermediate in the decarboxylation and oxidation reactions of the...

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Identifiers

PMID: 25526893
DOI: 10.1016/j.freeradbiomed.2014.12.012
PMCID: PMC4355160

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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