Article
Epifluorescence, SEM, TEM and nanoSIMS image analysis of the cold phenotype of Clostridium psychrophilum at subzero temperatures.
FEMS microbiology ecology - 1 Dec 2014
Perfumo Amedea, Elsaesser Andreas, Littmann Sten, Foster Rachel A, Kuypers Marcel M M, Cockell Charles S, Kminek Gerhard
Abstract excerpt
We have applied an image-based approach combining epifluorescence microscopy, electron microscopy and nanoscale secondary ion mass spectrometry (nanoSIMS) with stable isotope probing to examine directly the characteristic cellular features involved in the expression of the cold phenotype in the Antarctic bacterium Clostridium psychrophilum exposed to a temperature range from +5 to -15 °C under anoxic conditions....
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