Article
Molecular pathotyping and phylogenesis of the first Newcastle disease virus strain isolated from backyard chickens in Qatar.
Tropical animal health and production - 1 Jan 2015
Haroun Mohamed, Mohran Khalid Abdelhakeem, Hassan Mahmoud Mahmoud, Abdulla Nawal Mohamed
Abstract excerpt
The first Newcastle disease virus (NDV) field strain (NDV-QC08), previously isolated and identified in Qatar, was further subjected to molecular characterization and phylogenic analysis at passage levels two. Initial amplification and detection of the complementary DNA (cDNA) fragment of the NDV RNA amnioallantoic fluid (AAF) extract demonstrated the positive virus identity using real-time reverse transcriptase...
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