Article
An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants.
Journal of microbiological methods - 1 Nov 2014
Gillis Annika, Mahillon Jacques
Abstract excerpt
A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants.
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