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Article

[Establishment of an allele-specific PCR method for direct screening of CYP21A2 gene mutation].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Aug 2014

Zou Haiqiang, Liu Yan, Wang Weimin, Zhang Fenghuan, Zhao Baojian, Liang Junchao

Abstract excerpt

OBJECTIVE: To establish an allele-specific PCR method for detect screening of CYP21A2 gene mutation. METHODS: Allele-specific PCR primers and analogy primers were designed based on the sequence alignment of CYP21A2 and CYP21AP genes. Genomic DNA was extracted from blood specimens of 4 patients with 21-hydroxylase deficiency and 5 healthy controls and respectively amplified with allele-specific PCR primers and...

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Identifiers

PMID: 25119915
DOI: 10.3760/cma.j.issn.1003-9406.2014.04.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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