Article
Fast and cost-effective genetic mapping in apple using next-generation sequencing.
G3 (Bethesda, Md.) - 16 Jul 2014
Gardner Kyle M, Brown Patrick, Cooke Thomas F, Cann Scott, Costa Fabrizio, Bustamante Carlos, Velasco Riccardo, Troggio Michela, Myles Sean
Abstract excerpt
Next-generation DNA sequencing (NGS) produces vast amounts of DNA sequence data, but it is not specifically designed to generate data suitable for genetic mapping. Recently developed DNA library preparation methods for NGS have helped solve this problem, however, by combining the use of reduced representation libraries with DNA sample barcoding to generate genome-wide genotype data from a common set of genetic...
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