Article
Structure and cell wall cleavage by modular lytic transglycosylase MltC of Escherichia coli.
ACS chemical biology - 19 Sept 2014
Artola-Recolons Cecilia, Lee Mijoon, Bernardo-García Noelia, Blázquez Blas, Hesek Dusan, Bartual Sergio G, Mahasenan Kiran V, Lastochkin Elena, Pi Hualiang, Boggess Bill, Meindl Kathrin, Usón Isabel, Fisher Jed F, Mobashery Shahriar, Hermoso Juan A
Abstract excerpt
The lytic transglycosylases are essential bacterial enzymes that catalyze the nonhydrolytic cleavage of the glycan strands of the bacterial cell wall. We describe here the structural and catalytic properties of MltC, one of the seven lytic transglycosylases found in the genome of the Gram-negative bacterium Escherichia coli. The 2.3 Å resolution X-ray structure of a soluble construct of MltC shows a unique,...
Topics
- Catalytic Domain
- Cell Wall
- Cloning, Molecular
- Crystallography, X-Ray
- Escherichia coli
- Escherichia coli Proteins
- Glycosyltransferases
- Models, Molecular
- Mutation