Article
Detection and sequence analysis of grapevine virus B isolates from China.
Acta virologica - 1 Jan 2014
Hu G J, Dong Y F, Zhang Z P, Fan X D, Fang R, Zhu H J
Abstract excerpt
The presence of grapevine virus B (GVB) was detected in 188 grapevine samples from China by double antibody sandwich ELISA (DAS-ELISA) and reverse transcription-PCR (RT-PCR). The accuracy of detection by RT-PCR was confirmed by sequencing amplified PCR fragments. Seventeen samples were GVB-positive by DAS-ELISA and five by RT-PCR. The isolate QMW proved to be positive by RT-PCR only, and four isolates (DGWH, DGW,...
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