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First molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars and improvement of the RT-PCR detection assay.

Archives of virology - 1 Dec 2016

Fonseca Filomena, Duarte Vilma, Teixeira Santos Margarida, Brazão João, Eiras-Dias Eduardo

Abstract excerpt

This work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in...

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PMID: 27604120
DOI: 10.1007/s00705-016-3024-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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