Article
Reliable determination of training-induced alterations in muscle fiber composition in human skeletal muscle using quantitative polymerase chain reaction.
Scandinavian journal of medicine & science in sports - 1 Oct 2014
Ellefsen S, Vikmoen O, Zacharoff E, Rauk I, Slettaløkken G, Hammarström D, Strand T A, Whist J E, Hanestadhaugen M, Vegge G, Fagernes C E, Nygaard H, Hollan I, Rønnestad B R
Abstract excerpt
Determination of muscle fiber composition in human skeletal muscle biopsies is often performed using immunohistochemistry, a method that tends to be both time consuming, technically challenging, and complicated by limited availability of tissue. Here, we introduce quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based Gene-family profiling (GeneFam) of myosin heavy chain (MyHC) mRNA...
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