Article
Agrobacterium-derived cytokinin influences plastid morphology and starch accumulation in Nicotiana benthamiana during transient assays.
BMC plant biology - 9 May 2014
Erickson Jessica L, Ziegler Jörg, Guevara David, Abel Steffen, Klösgen Ralf B, Mathur Jaideep, Rothstein Steven J, Schattat Martin H
Abstract excerpt
BACKGROUND: Agrobacterium tumefaciens-based transient assays have become a common tool for answering questions related to protein localization and gene expression in a cellular context. The use of these assays assumes that the transiently transformed cells are observed under relatively authentic physiological conditions and maintain 'normal' sub-cellular behaviour. Although this premise is widely accepted, the...
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