Article
Rescue of syringyl lignin and sinapate ester biosynthesis in Arabidopsis thaliana by a coniferaldehyde 5-hydroxylase from Eucalyptus globulus.
Plant cell reports - 1 Aug 2014
García José Renán, Anderson Nickolas, Le-Feuvre Regis, Iturra Carolina, Elissetche Juan, Chapple Clint, Valenzuela Sofía
Abstract excerpt
KEY MESSAGE: The gene coding for F5H from Eucalyptus globulus was cloned and used to transform an f5h -mutant of Arabidopsis thaliana , which was complemented, thus verifying the identity of the cloned gene. Coniferaldehyde 5-hydroxylase (F5H; EC 1.14.13) is a cytochrome P450-dependent monooxygenase that catalyzes the 5-hydroxylation step required for the production of syringyl units in lignin biosynthesis. The...
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