Article
Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing.
Journal of microbiological methods - 1 May 2014
Monteiro Jussimara, Widen Raymond H, Pignatari Antonio C C, Kubasek Carly, Silbert Suzane
Abstract excerpt
A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.
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