Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay.

doi:10.1128/JCM.03437-13

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Article

Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay.

Journal of clinical microbiology - 1 May 2014

Tocqueville Véronique, Ferré Séverine, Nguyen Ngoc Hong Phuc, Kempf Isabelle, Marois-Créhan Corinne

Abstract excerpt

A real-time TaqMan PCR assay based on the gene encoding the protein p37 was developed to detect Mycoplasma hyorhinis. Its specificity was validated with 29 epidemiologically unrelated M. hyorhinis strains (28 field strains and one reference strain) and other mycoplasma species or with other microorganisms commonly found in pigs. The estimated detection limit of this qPCR assay was 125 microorganism...

Topics

Bacterial Proteins
Genes, Bacterial
Genotype
Multilocus Sequence Typing
Mycoplasma Infections
Mycoplasma hyorhinis
Phylogeny
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA

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Identifiers

PMID: 24622092
DOI: 10.1128/JCM.03437-13
PMCID: PMC3993664

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