Article
Validation of fragment analysis by capillary electrophoresis to resolve mixed infections by Cryptosporidium parvum subpopulations.
Parasitology research - 1 May 2014
Quílez Joaquín, Hadfield Stephen J, Ramo Ana, Vergara-Castiblanco Claudia, Chalmers Rachel M
Abstract excerpt
The potential of capillary electrophoresis (CE)-based DNA fragment analysis to identify mixed infections by Cryptosporidium parvum subpopulations was validated using high-resolution slab-gel electrophoresis. A selection of genomic DNA samples from C. parvum isolates with CE electropherogram profiles indicative of two concurrent alleles at one or more of six mini and microsatellite loci (MSB, MS5, ML1, ML2, TP14,...
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