Article
Comparison of multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for molecular subtyping of Cryptococcus neoformans. The Cryplococcal Disease Active Surveillance Group.
Journal of clinical microbiology - 1 Jul 1995
Brandt M E, Hutwagner L C, Kuykendall R J, Pinner R W
Abstract excerpt
We evaluated multilocus enzyme electrophoresis (MEE) and random amplified polymorphic DNA (RAPD) for their usefulness in subtyping 344 Cryptococcus neoformans clinical isolates obtained from four U.S. metropolitan areas in 1992 to 1994. MEE and RAPD with five primers both discriminated between the two varieties of C. neofromans. MEE divided C. neoformans var. neoformans isolates into 15 enzyme electrophoretic...
Topics
- Cryptococcus neoformans
- DNA Fingerprinting
- DNA, Fungal
- Electrophoresis
- Enzymes
- Evaluation Studies as Topic
- Genetic Variation
- Humans
- Polymorphism, Genetic
- Reproducibility of Results