Article
Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida).
Parasites & vectors - 3 Mar 2014
Lv Jizhou, Wu Shaoqiang, Zhang Yongning, Chen Yan, Feng Chunyan, Yuan Xiangfen, Jia Guangle, Deng Junhua, Wang Caixia, Wang Qin, Mei Lin, Lin Xiangmei
Abstract excerpt
BACKGROUND: The 5' region of cytochrome oxidase I (COI) is the standard marker for DNA barcoding. However, COI has proved to be of limited use in identifying some species, and for some taxa, the coding sequence is not efficiently amplified by PCR. These deficiencies lead to uncertainty as to whether COI is the most suitable barcoding fragment for species identification of ticks. METHODS: In this study, we...
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