Article
Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].
Molecular biology reports - 1 May 2014
An Xia, Wang Bo, Liu Lijun, Jiang Hui, Chen Jie, Ye Shengtuo, Chen Leiyu, Guo Pingan, Huang Xing, Peng Dingxiang
Abstract excerpt
In this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [Boehmeria nivea (L.) Gaud.] via Agrobacterium-mediated genetic transformation. Sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure....
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