Article
A high quality Arabidopsis transcriptome for accurate transcript-level analysis of alternative splicing.
Nucleic acids research - 19 May 2017
Zhang Runxuan, Calixto Cristiane P G, Marquez Yamile, Venhuizen Peter, Tzioutziou Nikoleta A, Guo Wenbin, Spensley Mark, Entizne Juan Carlos, Lewandowska Dominika, Ten Have Sara, Frei Dit Frey Nicolas, Hirt Heribert, James Allan B, Nimmo Hugh G, Barta Andrea, Kalyna Maria, Brown John W S
Abstract excerpt
Alternative splicing generates multiple transcript and protein isoforms from the same gene and thus is important in gene expression regulation. To date, RNA-sequencing (RNA-seq) is the standard method for quantifying changes in alternative splicing on a genome-wide scale. Understanding the current limitations of RNA-seq is crucial for reliable analysis and the lack of high quality, comprehensive transcriptomes...
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